Detect & Identify Microbes Within Minutes
Optical Precision Meets Molecular Certainty.
The GramRay MK3 is the first rapid microbio system to enable DeepMorph-AI directed Raman spectroscopy. Stop guessing at dots on a screen—start confirming molecular fingerprints down to the species level.
The Challenge: Real-World Samples Are Not Clean.
Whether testing cell therapies, viscous lotions, or turbid juices, background noise is the enemy of rapid methods. Non-viable particles and cell debris often trigger false positives in classical microscopy or Solid-Phase Cytometry.
The GramRay Difference: It doesn't just count; it verifies. By combining trained deep learning models with molecular vibration spectroscopic information, GramRay distinguishes true biological contaminants from inert particles with absolute certainty.
Versatile sample holder, and linear motor precision.
The doors open wide to give you full access – for changing sample formats, fine-tuning positions, or cleaning. The platform is designed for stability, minimal vibration, and effortless handling.
Close the doors. Press start. Done.
Once the samples are in, the GramRay MK3 takes over.
Laser-safe. Fully automated. No further user interaction needed. The system does the work – measuring, detecting, identifying.
Robust enough for routine. Smart enough for precision.
The Technology: A Dual-Stage Intelligence Flow
Step 1: High-Speed Scouting The system scans the membrane filter using Dark-Field and optionally Fluorescence. DeepMorph multimodal AI instantly locates potential biological candidates, effectively filtering out the majority of background debris and non-biological particles.
Result: High throughput and recovery, even in complex matrices.
Step 2: Molecular Verification (Raman) The system targets the identified candidates with a laser to capture their Raman Spectrum—a unique chemical fingerprint. If the particle doesn't have the right molecular signature, it is rejected.
Result: Zero false positives. Molecular proof of viability.
Step 3: Identification & Classification The spectral fingerprint is analyzed with advanced machine learning algorithms to determine the identity and physiological state of the microbe.
DeepMorph AI – Smarter Detection
DeepMorph identifies potential viable cells before Raman acquisition.
Its model, trained on mibiC’s proprietary illumination and filter data, directs the Raman probe precisely to relevant targets.
RAMANMETRIX™ – Learn Your Own Microbes
Create your own microbial fingerprint library.
Record one species for your RAMANMETRIX™ model in about one hour, then distinguish lab isolates from biofilm-derived strains in just 10 seconds per microbe.
Connect to the Biophotonics Diagnostics curated Raman Microbes Database for confirmation and updates.
Flexible Sample Preparation
No two samples are alike. GramRay accommodates both clear and complex matrices for reproducible results.
Choose the most efficient preparation path for your sample type:
- Filtration mode – for liquids and high-volume samples; cells are concentrated and measured directly on a metal-coated filter.
- Suspension mode – for colonies or unfilterable matrices; apply a 10 µL drop that dries in 5 min for immediate isolate ID or RamanMetrix™ training.
Each mode is optimized for throughput, recovery, and spectral clarity.
Tailored Analytical Modes
Different applications demand different detection strategies.
GramRay MK3 combines imaging, AI, and Raman spectroscopy in a single automated workflow:
- Autofluorescence Detection – differentiates biological from inert particles.
- DeepMorph AI Guidance – trained on hundreds of thousands of images, boosting viable-cell detection ×10.
- Multimodal Raman Analysis – merges dark-field, fluorescence, and Raman for maximum selectivity.
Effortless Mounting & Automation
Mount your sample with a snap. The GramRay MK3 automatically recognizes sample position and starts measurement at the push of a button.
Run a single membrane or up to eight samples in parallel with Auto Focus & Auto Scan walk-away routines that image the entire filter in minutes.
Pre-stored methods adjust parameters for bioburden, sterility, or IPC tasks, ensuring push-button reproducibility and full cGMP compliance.
Calibration & Self-Tests
- Autocalibration before every run
- Automatic self-tests for optical / analytical stability
- Includes positive & negative controls for verification
- Ensures traceable, validated performance
Illumination & Multimodality
Dark-Field Illumination — Enhances contrast by capturing only scattered light, allowing for the detection of faint or low-density microbes independent of the surrounding background.
Epi-Fluorescence | precise fluorescent imaging, enabling clear visualization of stained cells even in complex matrices.
Autofluorescence Detection | Identifies naturally fluorescent microbes without staining, allowing fast recognition of biological material amid non-biological particles.
Multimodal Integration | Combines dark-field, fluorescence, and Raman in one automated run, ensuring robust classification of target organisms while filtering out noise and debris.
Illumination+ Module | Offers six selectable light sources from UV to near-IR, providing optimal excitation for diverse fluorophores and supporting reliable detection across varied sample types.
From Sample to Certainty
Filter your liquid sample and detect microbial presence down to a single viable cell per mL.
Optimized filtration and high-sensitivity Raman analysis capture, count, and classify every organism within minutes.
From Colony to Clarity
Take a colony, resuspend, and spot.
Analyze up to 400 microbes per hour for instant identification and effortless database growth.
Regulatory readiness is built in.
- 21 CFR Part 11-compliant user management
- Audit trail & data integrity controls
- cGMP-compliant result generation (pass/fail output, taxonomy, viability)
- ISO 9001-certified manufacturing and full IQ/OQ/PQ documentation
GramRay is built to identify, not just detect. The system allows for species-level differentiation when trained on relevant libraries.
Proven Identification Power
Demonstrated Capability: As presented at the PDA Conference (Procter & Gamble study), the GramRay technology successfully differentiated over 120 USP reference strains down to the species level.
Customizable Intelligence: The system is designed to be trained on your facility’s specific isolates ("House Germs"), ensuring your release testing targets your specific risks.
Physiological Profiling (Viability & VBNC)
Standard culture methods often fail to detect Viable But Non-Culturable (VBNC) organisms. GramRay analyzes the spectral metabolic state (e.g. peak shifts indicating protein/lipid changes) to determine if a cell is:
Vital: Active metabolism.
Stressed / VBNC: Alive but dormant (often missed by plates).
Dead: Irreversibly damaged structure.
Intelligent Throughput
Scan Speed: Full filter imaging in 10 minutes.
Analysis Speed: Up to 400 verified identifications per hour.
Workflow: Automated calibration, focus, and scanning routines allow for "Load & Go" operation for up to 8 samples.